Surfactin and fengycin B extracted from Bacillus pumilus W-7 provide protection against potato late blight via distinct and synergistic mechanisms.
Identifieur interne : 000045 ( Main/Exploration ); précédent : 000044; suivant : 000046Surfactin and fengycin B extracted from Bacillus pumilus W-7 provide protection against potato late blight via distinct and synergistic mechanisms.
Auteurs : Youyou Wang [République populaire de Chine] ; Congying Zhang [République populaire de Chine] ; Jiao Liang [République populaire de Chine] ; Le Wang [République populaire de Chine] ; Wenbin Gao [République populaire de Chine] ; Jizhi Jiang [République populaire de Chine] ; Ruixue Chang [République populaire de Chine]Source :
- Applied microbiology and biotechnology [ 1432-0614 ] ; 2020.
Abstract
Potato late blight caused by Phytophthora infestans is one of the most serious plant diseases worldwide. Cyclic lipopeptides (CLPs) extracted from Bacillus strains exhibit a promising effect in the biocontrol of a variety of phytopathogens. However, the specific inhibitory effects and underlying mechanisms of CLPs against P. infestans are poorly understood. In this study, we showed that Bacillus pumilus W-7 can inhibit the growth of P. infestans mycelium. Two metabolites from W-7, surfactin and fengycin B, were identified using MS/MS. Fengycin B inhibited mycelium growth by inducing mycelium deformations, oxidative damage, and mitochondrial dysfunction. Surfactin induced potato plant defense responses by increasing the expression of the biocontrol genes (pod, pal, and cat) and their enzyme activities (POD, PAL, and CAT). Also, surfactin and fengycin B could exhibit a synergistic inhibitory effect on P. infestans. Taken together, our findings indicate that B. pumilus W-7 and its CLPs are potential environmentally friendly and effective biocontrol agents for the preservation of potato crops. KEY POINTS: • Lipopeptides of surfactin and fengycin B are extracted from Bacillus pumilus W-7. • Fengycin B inhibits Phytophthora infestans mycelium growth in a direct manner. • Surfactin induces potato plant defense responses to control late blight.
DOI: 10.1007/s00253-020-10773-y
PubMed: 32696296
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Surfactin and fengycin B extracted from Bacillus pumilus W-7 provide protection against potato late blight via distinct and synergistic mechanisms.</title><author><name sortKey="Wang, Youyou" sort="Wang, Youyou" uniqKey="Wang Y" first="Youyou" last="Wang">Youyou Wang</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Hebei University, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Hebei University, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Congying" sort="Zhang, Congying" uniqKey="Zhang C" first="Congying" last="Zhang">Congying Zhang</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Hebei University, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Hebei University, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Liang, Jiao" sort="Liang, Jiao" uniqKey="Liang J" first="Jiao" last="Liang">Jiao Liang</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Hebei University, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Hebei University, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Le" sort="Wang, Le" uniqKey="Wang L" first="Le" last="Wang">Le Wang</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Hebei University, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Hebei University, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Wenbin" sort="Gao, Wenbin" uniqKey="Gao W" first="Wenbin" last="Gao">Wenbin Gao</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Hebei University, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Hebei University, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Jiang, Jizhi" sort="Jiang, Jizhi" uniqKey="Jiang J" first="Jizhi" last="Jiang">Jizhi Jiang</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Hebei University, Baoding, China. jizhijiang909@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Hebei University, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Chang, Ruixue" sort="Chang, Ruixue" uniqKey="Chang R" first="Ruixue" last="Chang">Ruixue Chang</name><affiliation wicri:level="1"><nlm:affiliation>Hebei Jiuxin Testing Technology Co., Ltd., Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hebei Jiuxin Testing Technology Co., Ltd., Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32696296</idno><idno type="pmid">32696296</idno><idno type="doi">10.1007/s00253-020-10773-y</idno><idno type="wicri:Area/Main/Corpus">000124</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000124</idno><idno type="wicri:Area/Main/Curation">000124</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000124</idno><idno type="wicri:Area/Main/Exploration">000124</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Surfactin and fengycin B extracted from Bacillus pumilus W-7 provide protection against potato late blight via distinct and synergistic mechanisms.</title><author><name sortKey="Wang, Youyou" sort="Wang, Youyou" uniqKey="Wang Y" first="Youyou" last="Wang">Youyou Wang</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Hebei University, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Hebei University, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Congying" sort="Zhang, Congying" uniqKey="Zhang C" first="Congying" last="Zhang">Congying Zhang</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Hebei University, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Hebei University, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Liang, Jiao" sort="Liang, Jiao" uniqKey="Liang J" first="Jiao" last="Liang">Jiao Liang</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Hebei University, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Hebei University, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Le" sort="Wang, Le" uniqKey="Wang L" first="Le" last="Wang">Le Wang</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Hebei University, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Hebei University, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Wenbin" sort="Gao, Wenbin" uniqKey="Gao W" first="Wenbin" last="Gao">Wenbin Gao</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Hebei University, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Hebei University, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Jiang, Jizhi" sort="Jiang, Jizhi" uniqKey="Jiang J" first="Jizhi" last="Jiang">Jizhi Jiang</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Hebei University, Baoding, China. jizhijiang909@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Hebei University, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Chang, Ruixue" sort="Chang, Ruixue" uniqKey="Chang R" first="Ruixue" last="Chang">Ruixue Chang</name><affiliation wicri:level="1"><nlm:affiliation>Hebei Jiuxin Testing Technology Co., Ltd., Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hebei Jiuxin Testing Technology Co., Ltd., Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author></analytic><series><title level="j">Applied microbiology and biotechnology</title><idno type="eISSN">1432-0614</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Potato late blight caused by Phytophthora infestans is one of the most serious plant diseases worldwide. Cyclic lipopeptides (CLPs) extracted from Bacillus strains exhibit a promising effect in the biocontrol of a variety of phytopathogens. However, the specific inhibitory effects and underlying mechanisms of CLPs against P. infestans are poorly understood. In this study, we showed that Bacillus pumilus W-7 can inhibit the growth of P. infestans mycelium. Two metabolites from W-7, surfactin and fengycin B, were identified using MS/MS. Fengycin B inhibited mycelium growth by inducing mycelium deformations, oxidative damage, and mitochondrial dysfunction. Surfactin induced potato plant defense responses by increasing the expression of the biocontrol genes (pod, pal, and cat) and their enzyme activities (POD, PAL, and CAT). Also, surfactin and fengycin B could exhibit a synergistic inhibitory effect on P. infestans. Taken together, our findings indicate that B. pumilus W-7 and its CLPs are potential environmentally friendly and effective biocontrol agents for the preservation of potato crops. KEY POINTS: • Lipopeptides of surfactin and fengycin B are extracted from Bacillus pumilus W-7. • Fengycin B inhibits Phytophthora infestans mycelium growth in a direct manner. • Surfactin induces potato plant defense responses to control late blight.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32696296</PMID><DateRevised><Year>2020</Year><Month>08</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-0614</ISSN><JournalIssue CitedMedium="Internet"><Volume>104</Volume><Issue>17</Issue><PubDate><Year>2020</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Applied microbiology and biotechnology</Title><ISOAbbreviation>Appl Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Surfactin and fengycin B extracted from Bacillus pumilus W-7 provide protection against potato late blight via distinct and synergistic mechanisms.</ArticleTitle><Pagination><MedlinePgn>7467-7481</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00253-020-10773-y</ELocationID><Abstract><AbstractText>Potato late blight caused by Phytophthora infestans is one of the most serious plant diseases worldwide. Cyclic lipopeptides (CLPs) extracted from Bacillus strains exhibit a promising effect in the biocontrol of a variety of phytopathogens. However, the specific inhibitory effects and underlying mechanisms of CLPs against P. infestans are poorly understood. In this study, we showed that Bacillus pumilus W-7 can inhibit the growth of P. infestans mycelium. Two metabolites from W-7, surfactin and fengycin B, were identified using MS/MS. Fengycin B inhibited mycelium growth by inducing mycelium deformations, oxidative damage, and mitochondrial dysfunction. Surfactin induced potato plant defense responses by increasing the expression of the biocontrol genes (pod, pal, and cat) and their enzyme activities (POD, PAL, and CAT). Also, surfactin and fengycin B could exhibit a synergistic inhibitory effect on P. infestans. Taken together, our findings indicate that B. pumilus W-7 and its CLPs are potential environmentally friendly and effective biocontrol agents for the preservation of potato crops. KEY POINTS: • Lipopeptides of surfactin and fengycin B are extracted from Bacillus pumilus W-7. • Fengycin B inhibits Phytophthora infestans mycelium growth in a direct manner. • Surfactin induces potato plant defense responses to control late blight.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Youyou</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Life Science, Hebei University, Baoding, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Congying</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>College of Life Science, Hebei University, Baoding, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Jiao</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>College of Life Science, Hebei University, Baoding, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Le</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>College of Life Science, Hebei University, Baoding, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Wenbin</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>College of Life Science, Hebei University, Baoding, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Jizhi</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>College of Life Science, Hebei University, Baoding, China. jizhijiang909@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Ruixue</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Hebei Jiuxin Testing Technology Co., Ltd., Baoding, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>201303018</GrantID><Agency>Agriculture Special Scientific Research Program of China</Agency><Country></Country></Grant><Grant><GrantID>C2015201231</GrantID><Agency>Natural Science Foundation of Hebei Province</Agency><Country></Country></Grant><Grant><GrantID>1911F001</GrantID><Agency>Research Program of Baoding Bureau of Science and Technology</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>07</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Appl Microbiol Biotechnol</MedlineTA><NlmUniqueID>8406612</NlmUniqueID><ISSNLinking>0175-7598</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Bacillus pumilus</Keyword><Keyword MajorTopicYN="N">Fengycin B</Keyword><Keyword MajorTopicYN="N">Inhibition</Keyword><Keyword MajorTopicYN="N">Phytophthora infestans</Keyword><Keyword MajorTopicYN="N">Potato late blight</Keyword><Keyword MajorTopicYN="N">Surfactin</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>03</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>07</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>06</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>7</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>7</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32696296</ArticleId><ArticleId IdType="doi">10.1007/s00253-020-10773-y</ArticleId><ArticleId IdType="pii">10.1007/s00253-020-10773-y</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Youyou" sort="Wang, Youyou" uniqKey="Wang Y" first="Youyou" last="Wang">Youyou Wang</name></noRegion><name sortKey="Chang, Ruixue" sort="Chang, Ruixue" uniqKey="Chang R" first="Ruixue" last="Chang">Ruixue Chang</name><name sortKey="Gao, Wenbin" sort="Gao, Wenbin" uniqKey="Gao W" first="Wenbin" last="Gao">Wenbin Gao</name><name sortKey="Jiang, Jizhi" sort="Jiang, Jizhi" uniqKey="Jiang J" first="Jizhi" last="Jiang">Jizhi Jiang</name><name sortKey="Liang, Jiao" sort="Liang, Jiao" uniqKey="Liang J" first="Jiao" last="Liang">Jiao Liang</name><name sortKey="Wang, Le" sort="Wang, Le" uniqKey="Wang L" first="Le" last="Wang">Le Wang</name><name sortKey="Zhang, Congying" sort="Zhang, Congying" uniqKey="Zhang C" first="Congying" last="Zhang">Congying Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000045 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000045 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32696296
|texte= Surfactin and fengycin B extracted from Bacillus pumilus W-7 provide protection against potato late blight via distinct and synergistic mechanisms.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32696296" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |